What is the expected size range of Covaris-sheared cDNA after library preparation?
What is the expected size range of Covaris-sheared cDNA after library preparation?
cDNA generated with a SMARTer Ultra low kit that is sheared using Covaris technology and prepared with the Low Input Library Prep Kit typically has a size distribution of 150–600 bp with a peak at approximately 250–300 bp.Example Bioanalyzer electropherogram of a cDNA library prepared for Illumina sequencing. The cDNA library was prepared from 10 pg of Mouse Brain Total RNA using a SMARTer Ultra low kit and the Low Input Library Prep Kit.