Can I use indexing primers from vendors other than Takara Bio USA?
Can I use indexing primers from vendors other than Takara Bio USA?
Indexing primers from vendors other than Takara Bio USA, are not compatible with the SMARTer® Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian, since within the workflow of the SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian, PCR incorporation of indexing primers requires proprietary overlaps with the first-strand cDNA template.