What is the mechanism of the RNA fragmentation method used in the workflow of the SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian?
What is the mechanism of the RNA fragmentation method used in the workflow of the SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian?
SMARTer Stranded v2 Pico protocol is using RNA heat fragmentation in the presence of Mg++ included in the 5X First -Strand Buffer: fragmentation is stopped by placing the reaction mix on ice for 2 minutes.