What is the mechanism of the RNA fragmentation method used in the workflow of the SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian?

What is the mechanism of the RNA fragmentation method used in the workflow of the SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian?

SMARTer Stranded v2 Pico protocol is using RNA heat fragmentation in the presence of Mg++ included in the 5X First -Strand Buffer: fragmentation is stopped by placing the reaction mix on ice for 2 minutes.

Stranded RNA-seq for mammalian samples

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