When can the resin no longer be used?
When can the resin no longer be used?
The resin can no longer be used if it turns opaque/milky white, if it turns brown, if it loses its pink color, or if the binding capacity drops too low. These changes are usually due to using incompatible reagents on the column or regenerating the resin too many times. This can also happen if the entire protein precipitates onto the column. The researcher may want to remove the precipitated material by adding buffer with a denaturant, but we do not have specific protocols for this. The best recommendation is to use fresh resin.