What precautions should be taken when working with recombinant AAV?
What precautions should be taken when working with recombinant AAV?
It is imperative to fully understand the potential hazards of and necessary precautions for the laboratory use of AAV vectors. Viruses could, depending on your gene insert, be potentially hazardous. Similar vectors have been approved for human gene therapy trials, attesting to their potential ability to express genes in vivo. For these reasons, due caution must be exercised in the production and handling of any recombinant viruses.
Follow all applicable guidelines for research involving recombinant DNA. Take appropriate safety measures when producing or handling recombinant AAV, including working in a biological safety cabinet and wearing protective laboratory coats, face protection, and gloves.