Can I use the Unique Dual index kit from cat No R400745 with SMART-seq mRNA LP kit?
Can I use the Unique Dual index kit from cat No R400745 with SMART-seq mRNA LP kit?
No - R400745 includes 5uM UDIs (Catalog numbers 631752 - 634756) . The Unique Dual Index Kit used in the SMART-Seq mRNA LP (with UMIs) kit has 12.5 uM UDIs, and the Iibrary construction protocol in the SMART-Seq mRNA LP (with UMIs) User Manual is written for 12.5 uM UDIs (Catalog numbers 634752 - 634756) Unique Dual Index Kit