Does Xfect Protein Transfection Reagent deliver a high amount of protein?

Yes, Xfect Protein Transfection Reagent delivers far more protein per cell than the leading protein transfection reagent (Product C), a peptide-based reagent, in a broad range of cell types. This was demonstrated when we compared the amounts of ?-galactosidase and fluorescent proteins delivered by each reagent. When we compared six different cell types, Xfect protein delivered 3- to 13-fold more protein than Product C. The biggest disadvantage of other protein delivery reagents is their limited ability to allow monitoring of a protein's biological effects (due to an inability to deliver sufficient active protein). Xfect protein delivers more protein per cell than other reagents, so it should expand the applications for protein delivery and/or allow you to use less protein for transfection.

Xfect Protein Transfection Reagent delivers more recombinant fluorescent proteins than Product C into a range of cell types. Xfect Protein Transfection Reagent and Product C were used to transfect a number of cell lines with purified recombinant fluorescent protein (either rAcGFP1 or rDsRed-Express). In all of the cell lines tested, a far greater MFI (mean fluorescence intensity) was detected via flow cytometry after transfection with Xfect Protein Transfection Reagent than after transfection with Product C.

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