For how many passages can the DE cells be maintained? Can the DE cells be scaled up?
For how many passages can the DE cells be maintained? Can the DE cells be scaled up?
The DE cells should have proliferative capacity; Hannan et al. 2013 described a protocol for expanding DE cells/foregut endoderm derived with their DE differentiation protocol. However, we haven't tried it on the DE cells derived with our differentiation protocol, and we do not have any medium for maintenance/expansion of the DE cells.
Reference
Hannan, N. R. F. et al. Generation of Multipotent Foregut Stem Cells from Human Pluripotent Stem Cells. Stem Cell Reports 1, 293–306 (2013).