Can I use AMPure bead purification for PCR -amplfied SMARTer smRNA-Seq instead of NucleoSpin Gel and PCR Clean-Up kit?
Can I use AMPure bead purification for PCR -amplfied SMARTer smRNA-Seq instead of NucleoSpin Gel and PCR Clean-Up kit?
We recommend NucleoSpin Gel and PCR Clean-Up kit for the purification of PCR-amplified SMARTer smRNA library because the 100ul PCR reaction volume is rather large , which makes AMPure bead purification challenging and can result in a suboptimal bead: sample separation." ProdCompatibility
What is the cumulative size of adapters incorporated in the SMARTer smRNA library? The combined length of 5’ and 3’ library adapters is 153 bp.