What are the input requirements for the SMARTer Human TCR a/b Profiling Kit v2?
What are the input requirements for the SMARTer Human TCR a/b Profiling Kit v2?
SMARTer Human TCR a/b Profiling Kit v2 utilizing oligo(dT) priming for the first-strand cDNA synthesis is compatible with high integrity total RNA (RIN>7) using 10ng-1ug obtained from peripheral blood leukocytes, 20 ng to 200 ng of total RNA obtained from whole blood, 1 ng to 100 ng of total RNA obtained from T cells, or with 1,000 to 10,000 purified, whole T cells.