Can I amplify TCR alpha and beta chains in one reaction?
Can I amplify TCR alpha and beta chains in one reaction?
TCR alpha and beta chains can be amplified in the same PCR2 reaction mix as described in the SMARTer Human TCR a/b Profiling Kit v2 User Manual V. Protocols - B. Protocol: TCR a/b Amplification and Sequencing Library Generation by Semi-Nested PCR - 2. PCR 2. If achieving equal coverage for each subunit is critical to your experiments, we recommend performing the reactions in PCR2 separately.