Can I amplify TCR alpha and beta chains separately?
Can I amplify TCR alpha and beta chains separately?
TCR alpha and beta chains can be amplified separately – each in a separate PCR2 reaction as described in the SMARTer Human TCR a/b Profiling Kit v2 User Manual V. Protocols - B. Protocol: TCR a/b Amplification and Sequencing Library Generation by Semi-Nested PCR - 2. PCR 2.