Can I load on the same lane of Illumina sequencer SMARTer® Human BCR IgG IgM H/K/L cDNA libraries together with libraries prepared by alternative methods?
Can I load on the same lane of Illumina sequencer SMARTer® Human BCR IgG IgM H/K/L cDNA libraries together with libraries prepared by alternative methods?
We usually do not recommend loading SMARTer BCR libraries with libraries prepared by other methods, since the average size of the SMARTer BCR library is 600-800bp, while the average size of most of Illumina libraries prepared by other methods is about 300bp. It is imperative that all the libraries are pooled in equimolar quantities (with compatible indexes) – otherwise libraries with smaller average size will cluster more efficiently over-representing SMARTer BCR libraries in the sequencing reads.