What additional kits are needed for preparing NGS libraries downstream of screening?
What additional kits are needed for preparing NGS libraries downstream of screening?
The Guide-it CRISPR Genome-Wide sgRNA Library NGS Analysis Kit (Cat. # 632647) enables preparation of Illumina-ready NGS libraries from cell populations screened using the Guide-it CRISPR Genome-Wide sgRNA Library System (Cat. # 632646), allowing for identification of sgRNA sequences and corresponding gene knockouts that have increased or decreased in frequency under the conditions of the screen. The kit provides all components necessary to prepare 10 sequencing libraries (20 PCR amplifications), including a genomic DNA purification kit, primers, PCR enzyme and buffers, and a PCR cleanup kit.