Where can I find more information about CRISPR/Cas9-mediated gene knockout?
Where can I find more information about CRISPR/Cas9-mediated gene knockout?
To learn more about CRISPR/Cas9 and the product solutions that we provide, please visit the following pages:
Gene editing learning center: https://www.takarabio.com/learning-centers/gene-function/gene-editing
Gene editing product catalog: https://www.takarabio.com/products/gene-function/gene-editing
The CRISPR/Cas9 system—a simple, RNA-programmable method to mediate genome editing in mammalian cells. CRISPR/Cas9 gene editing technology uses two key components that form a complex: Cas9 endonuclease and a single guide RNA (sgRNA) that directs Cas9 to a specific target site in genomic DNA. Cas9 cleaves the genomic DNA at the target site, which is then repaired by the error-prone non-homologous end joining (NHEJ) pathway, often leading to the creation of indels which can yield knockout phenotypes. CRISPR is very well suited for genome-wide knockout screens due to the ease of generating guide RNAs and the specificity of Cas9-sgRNA complexes to completely knockout the expression of target genes.