Can I validate the size distribution of the DNA SMART ChIP libraries by garose gel?
Can I validate the size distribution of the DNA SMART ChIP libraries by garose gel?
Agarose gel lacks the sensitivity for the validation of the DNA SMART ChIP libraries. Agarose gel lacks the sensitivity for the validation of the DNA SMART ChIP libraries. To minimize biases, ChIP-seq libraries are amplified at a minimal optimized number PCR cycle number, sufficient to generate library yield for Illumina sequencing while avoiding unnecessary overamplification.