Is it necessary to use indexing reagents for ThruPLEX DNA-Seq Kit? If so, which indexes should be used together for multiplexing?
Is it necessary to use indexing reagents for ThruPLEX DNA-Seq Kit? If so, which indexes should be used together for multiplexing?
Yes, it necessary to use indexing reagents, which must be purchased separately. They are supplied in tubes or a microplate and consist of amplification primers containing Illumina-compatible indexes, which must be used in the ThruPLEX DNA-Seq Library Amplification Reaction.