Can the ThruPLEX DNA-Seq Kit be used without a real-time PCR machine? If so, how do I know when to stop my run?
Can the ThruPLEX DNA-Seq Kit be used without a real-time PCR machine? If so, how do I know when to stop my run?
Yes, the ThruPLEX DNA-Seq Kit can be used even if a lab does not have access to a real-time PCR machine. Please refer to the ThruPLEX DNA-Seq Kit User Manual, which contains a table that provides a guide for selecting the number of PCR cycles based on the DNA input amount used.