Are you aware if PicoPLEX amplicons being blocked at the 5’ ends to prevent the amplified WGA from ligation?
Are you aware if PicoPLEX amplicons being blocked at the 5’ ends to prevent the amplified WGA from ligation?
The 5’ ends of PicoPLEX WGA amplicons are not 5’ blocked. However, some of the products might still be partially single-stranded, so it is very likely that some adapters would not ligate to these products.