What range of nuclei recovery rate should I expect with the Trekker kit?
What range of nuclei recovery rate should I expect with the Trekker kit?
End-to-end nuclei recovery, from tissue dissociation to positioning, depends mainly on three factors:
1. Recovery rate from nuclei dissociation—typically 50-70%
2. Recovery rate from the single-cell sequencing platform—typically 50-70% for the most efficient platforms, but varies by platform
3. Positioning rate by Trekker spatial barcodes—typically 60-90% nuclei positioned with at least one coordinate
The stacked yield averages at ~30%, or a range of 15–44%, recovery of the estimated number of intact nuclei in the tissue section.