How does SMART-Seq Stranded cDNA sequencing metrics correlate between RNA and cell inputs?
How does SMART-Seq Stranded cDNA sequencing metrics correlate between RNA and cell inputs?
SMART-Seq Stranded cDNA synthesis shows a good sequencing metrics correlation between cells and purified RNA inputs.
Libraries were generated using 10 pg of Control Total RNA (human brain) (Panels A and C) or a no-RNA control (Panel B) using the ultra-low workflow. Panel D shows an example of libraries generated directly from either single cells or a no-template control using the ultra-low workflow. Note that the no-template control exhibits a small amount of background <200 bp, and typically shows some amount of PCR product between 200 and 1,000 bp, in addition to a larger amount of product >1,000 bp. This background is typically unavoidable but is acceptable as long as libraries from RNA samples contain >3 times more material than no-template controls.