What are the input requirements for the SMARTer Human BCR IgG IgM H/K/L Profiling Kit?
What are the input requirements for the SMARTer Human BCR IgG IgM H/K/L Profiling Kit?
SMARTer Human BCR IgG IgM H/K/L Profiling Kit utilizing oligo(dT) priming for the first-strand cDNA synthesis is compatible with high integrity total RNA (RIN>7) using 10 ng to 1 µg of total RNA from peripheral blood mononuclear cells (PBMCs) , 1 to 100 ng of total RNA from B cells, no less than 100ng Whole Blood RNA (the protocol may require adjustments)