Can I amplify BCR IgG/IgM/IgK/IgL chains in one reaction?
Can I amplify BCR IgG/IgM/IgK/IgL chains in one reaction?
For optimal balance between the different chains, we recommend amplifying them separately. If multiplex PCR is preferred, in order to amplify all four chains at the same time, we recommend combining only heavy chain primers (G+M) and light chain primers (K+L) strting with PCR1- see the SMARTer® Human BCR IgG IgM H/K/L Profiling Kit User Manual -Appendix A: Guidelines for Multiplexing Heavy Chains (IgG + IgM) and Light.
Chains (IgK + IgL)