What are the benefits of using the fourth-generation SMART-Seq v4 kit versus other ultra-low input mRNA-seq kits?
What are the benefits of using the fourth-generation SMART-Seq v4 kit versus other ultra-low input mRNA-seq kits?
The SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing improves on our previous SMARTer Ultra low kits and outperforms both previously published protocols (including the SMART-Seq2 method) and existing kits. The SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing builds on our experience from three previous generations of SMARTer Ultra low kits, and the work done by Rickard Sandberg's group at Ludwig Cancer Research on the SMART-Seq2 method. This kit delivers the highest number of genes identified, maintains sequencing platform compatibility, and provides improved data for GC-rich transcripts from 1–1,000 intact cells (or 10 pg–10 ng of total RNA). The SMART-Seq v4 kit does this by incorporating the novel application of LNA technology used by the Ludwig team as well as innovations developed by Takara Bio.