What kit is recommended for degraded total RNA samples?
What kit is recommended for degraded total RNA samples?
For 200 pg–10 ng of degraded or nonpolyadenylated RNA samples we recommend the SMARTer Universal Low Input RNA Kit for Sequencing (Cat. # 634938). This kit is compatible with low-quality total RNA (RIN 2–3) such as that obtained from LCM or FFPE samples. As with the SMARTer stranded kit, the SMARTer Universal Low Input RNA Kit for Sequencing is a random-primed kit; therefore, total RNA samples must be depleted of rRNA. The cDNA generated with this kit is compatible with either Illumina or Ion Torrent sequencing platforms.