What media have been tested for compatibility with direct cDNA synthesis from intact mammalian cells?
What media have been tested for compatibility with direct cDNA synthesis from intact mammalian cells?
It is important to collect cells using media and buffers that do not suppress cDNA synthesis. PBS buffer has been tested and is compatible with all SMARTer Ultra low kits at all inputs (1–9 µl).
The following media have not been tested in-house; however, they have been externally validated for use with low-input volumes (1 µl).
SuperBlock (Pierce, Cat. # 37515)
0.1 ml of DMEM/F-12, GlutaMAX (Thermo Fisher Scientific, Cat. # 10565) + 3.6 µl of 25% BSA (Thermo Fisher Scientific, Cat. # A10008-01)
Note: For the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing and the SMARTer Ultra Low Input RNA Kit for Sequencing - v3, we have only tested PBS, other media were not tested with these kits.