After transduction of cells using RetroNectin reagent, what method do you recommend to resuspend the cells?
After transduction of cells using RetroNectin reagent, what method do you recommend to resuspend the cells?
For cell lines such as HL60 cells and K562 cells, we have verified that cells can be easily collected by pipetting from the RetroNectin reagent-coated dish. However, for several other cell lines, it may be difficult to collect the cells by pipetting without trypsin treatment.
In general, if cells cannot be collected by pipetting, we recommend collecting cells by trypsin treatment 24 hours after transduction and transferring the collected cells into a fresh dish. Then culture the cells in suspension for ~2 days, and collect the suspended cells for flow cytometry.