How do I split the 15-nt homologous overlap among adjacent DNA fragments?

How do I split the 15-nt homologous overlap among adjacent DNA fragments?

The homologous 15-nt overlap can be split between two adjacent DNA fragments. It can only be split between an insert and vector if the vector is linearized via inverse PCR.
Primer design for this option is not facilitated by the online Primer Design Tool. We recommend SnapGene Viewer as a helpful, free online tool to help with this task.

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