Can I use indexing primers from vendors other than Takara Bio USA?
Can I use indexing primers from vendors other than Takara Bio USA?
Indexing primers from vendors other than Takara Bio USA, are not compatible with the SMARTer® Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian, since within the workflow of the SMARTer Stranded Total RNA-Seq Kit v3 - Pico , PCR incorporation of indexing primers requires proprietary overlaps with the first-strand cDNA template as shown in the SMARTer® Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian User Manual - Figure 2. Schematic of technology in the SMARTer Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian