How can I decontaminate if I have PCR contamination?
-Leave pipettes under UV light in the cell culture hood overnight. UV irradiation promotes cross-linking of thymidine residues, damaging residual DNA.
-Spray workstations/equipment/pipettes with 10% bleach and then wipe clean.
-Change workstations; move the pre-PCR area to another pre-cleaned location.
-Do not use any instruments or pipettes you have used before.
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