Does cell morphology change with different culturing systems?
Does cell morphology change with different culturing systems?
| Yes. Scientists at Takara Bio have generated some interesting FACS data showing that iPS cells that were transferred from DEF-CS to competitor medium (E8, mTeSR, StemFit) changed back to the pattern that is originally observed in each medium. And they showed similar results for cells originally cultured in competitor media and then transferred to DEF-CS. So this flexibility should encourage customers that if they already have protocols for colonies or for differentiation, that they should be able to transition freely between medias. The caveat is that for any change in culture conditions, the cells may require a couple of passages to fully recover. And this amount of time in culture could vary depending on the competitor media. |