How do ssODNs differ from ssDNA? When is it better to use one vs. the other?
How do ssODNs differ from ssDNA? When is it better to use one vs. the other?
Single-stranded oligo deoxynucleotides (ssODNs) are produced routinely via chemical synthesis and can be used for engineering smaller edits (e.g., single nucleotide substitutions or shorter insertions <50 nt) as HDR templates with a total maximum length of 200 nt. For engineering larger insertions that necessitate HDR templates >500 nt, we recommend the use of ssDNA that can be produced with the Guide-it Long ssDNA Production System.