Can I perform paired-end sequencing of the DNA SMART ChIP library?
Can I perform paired-end sequencing of the DNA SMART ChIP library?
Single-end sequencing with Read 1 is generally sufficient for ChIP-seq. However, if paired-end sequencing is desired, we recommend using the DNA SMART Custom Read2 Seq Primer provided in the kit, that will allow sequencing to start right after the priming site of the DNA SMART Poly(dA) Primer, thus avoiding reading through the A/T tail created on the 3’ end of ssDNA molecules. Sequencing through the stretch of adenine bases may result in low sequencing performance due to low complexity" "ProdProtocol, ProdDescription"