What concentration of PicoPLEX Gold Single Cell DNA-Seq library should be loaded onto the Illumina MiSeq flow cell?
What concentration of PicoPLEX Gold Single Cell DNA-Seq library should be loaded onto the Illumina MiSeq flow cell?
With libraries made from a single cell using the PicoPLEX Gold Single Cell DNA-Seq Kit, a good starting point is to consider a library size of 300 bp and load 16 pM on the MiSeq® v3. Other platforms will have different loading concentrations, so we recommend following the manufacturers' instructions. It is very important to add at least 5% PhiX DNA to the library prior to loading on the flow cell to achieve optimal diversity.