What is the expected yield for the amplified library from the ThruPLEX DNA-Seq Kit?
What is the expected yield for the amplified library from the ThruPLEX DNA-Seq Kit?
The amount of amplified library can range from 100 ng to 1 µg depending upon many variables, including sample type, fragmentation size, and thermal cycler used. When starting with Covaris-fragmented reference DNA with an average size of 200 bp (using the recommended number of amplification cycles in the ThruPLEX DNA-Seq Kit User Manual), typical yields range from 300 to 700 ng.