When considering drop-out rates, does FACS lead to a significant percentage of empty wells in 384-well plates?
When considering drop-out rates, does FACS lead to a significant percentage of empty wells in 384-well plates?
When looking at drop-out rates, there are a few things we need to take into consideration.
First, is the potential that the sorter may not successfully place a single cell in each well. From testing at our flow cytometry core facility, we expect less than 10 empty wells in each 384-well plate.
Another thing to consider is negative controls. In our 384-well plates, we include three negative controls that are leading to, in part, that 1% drop-out rate.
Lastly, there could be a potential issue with the genomics portion of the library prep. Altogether, we believe a 1% drop-out rate for the total workflow is very reasonable.