What's the mechanism of Tet-inducible expression?
What's the mechanism of Tet-inducible expression?
Each Tet-On 3G inducible gene expression system includes two core elements:
1. The inducible PTRE3G promoter, which provides the lowest basal expression of any tetracycline-inducible promoter in the absence of bound transactivator. PTRE3G retains very strong induced expression when activated by transactivator binding.
2. The Tet-On 3G transactivator, which binds to and activates expression from TRE promoters in the presence of doxycycline (Dox). The Tet-On 3G system is optimized for expression in human cells and for sensitivity to Dox.