What is the mechanism of sequencing library construction using the SMART-Seq® Stranded Kit?
What is the mechanism of sequencing library construction using the SMART-Seq® Stranded Kit?
The workflow of the SMART-Seq® Stranded Kit compatible with 10pg-10ng total mammalian RNA or 1-1,000 whole intact cells, utilizes a random priming for the first-strand cDNA synthesis and incorporates the removal of rRNA sequences, generating Illumina-ready library representing coding and non-coding RNAs with strand information