Can I use a pooling option for the SMART-Seq Stranded cDNAs, generated from bulk RNA inputs?"
Can I use a pooling option for the SMART-Seq Stranded cDNAs, generated from bulk RNA inputs?"
No, one can pool only SMART-Seq Stranded cDNAs generated from single cells. SMART-Seq Stranded pooling is beneficial for single cells, since cell to cell variation is lower than that between bulk RNAs of variable quality. cDNAs generated from bulk RNAs of variable quality will have variable cDNA yield and size distribution, that is not feasible to predict or control.