Is SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian compatible with LCM and FFPE/ PFA cells?
Is SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian compatible with LCM and FFPE/ PFA cells?
SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian is not compatible with cDNA synthesis directly from FFPE/PFA or LCM cells. Fixated cells usually contain high level of single-stranded DNA which can be randomly primed and used as a template by the reverse transcriptase, thus incorporating genomic DNA sequences into the final cDNA library. RNA isolated from fixated cells (FFPE/PFA RNA has to be decrosslinked) can be used as input for the SMARTer Stranded v2 - Pico cDNA synthesis.