Do I need to use a PhiX DNA spike-in during the sequencing of the SMARTer Stranded Kit v2 - Pico cDNA libraries?
Do I need to use a PhiX DNA spike-in during the sequencing of the SMARTer Stranded Kit v2 - Pico cDNA libraries?
Illumina recommends the systematic inclusion of ~1% PhiX DNA to help assess the sequencing run performance and troubleshooting. If performing paired-end sequencing on two-channel Illumina sequencer consider adding 5-10% PhiX.