Do I need to use a PhiX DNA spike-in during the sequencing of the libraries, generated by the SMARTer Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian?
Do I need to use a PhiX DNA spike-in during the sequencing of the libraries, generated by the SMARTer Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian?
Illumina recommends the systematic inclusion of ~1% PhiX to help assess run performance and troubleshooting. For Illumina sequencers with patterned flow cells, include 10% PhiX spike-in. Follow Illumina guidelines on how to denature, dilute, and combine a PhiX control library with your own pool of libraries.