What method of RNA fragmentation is used in the SMARTer Stranded Total RNA-Seq Kit v3 - Pico workflow?
What method of RNA fragmentation is used in the SMARTer Stranded Total RNA-Seq Kit v3 - Pico workflow?
RNA is heat-fragmented in the presence of Mg2+ (included in the 5X First-Strand Buffer). Fragmentation is then stopped by placing the reaction mixture on ice for 2 minutes.