Which polymerases generate blunt ends versus A-overhangs?
Which polymerases generate blunt ends versus A-overhangs?
-Enzymes in the PrimeSTAR series
These enzymes exhibit substantial 3'?5' exonuclease activity and primarily generate amplification products with blunt ends. Therefore, we recommend using the Mighty Cloning Reagent Set (Blunt End) for blunt-end cloning.
-Taq and Terra enzymes
Takara Taq, Takara Ex Taq, Takara LA Taq, SpeedSTAR HS, EmeraldAmp, SapphireAmp Fast, and Terra PCR DNA polymerases primarily yield amplification products containing 3'-dA overhangs that can be directly used for TA-cloning. Blunt-end cloning is also possible using the Mighty Cloning Reagent Set (Blunt End).
-All Takara Bio DNA polymerases
The fragment terminal structure after amplification with a particular polymerase is indicated in the table below. Some polymerases generate a mixture of blunt-end and A-overhang products; other polymerases generate only blunt-end or A-overhang products.