Takara enzyme with highest fidelity
Takara enzyme with highest fidelity
PrimeSTAR polymerases provide better fidelity than Pfu DNA polymerase, which is widely considered to be a high-fidelity enzyme. PrimeSTAR Max DNA Polymerase has the highest fidelity; when this enzyme was used to amplify the entire pUC119 plasmid, sequence analysis detected only four mutations out of 370,656 total bases sequenced (an error rate of 0.0010%). In addition, compared to other enzymes, PrimeSTAR Max DNA Polymerase replicates repeat sequences with markedly better fidelity and exhibits a lower rate of template exchange (formation of chimeric molecules) with analogous sequences.