What is the recommended fluorescent dye to monitor amplification using a real-time PCR cycler?

What is the recommended fluorescent dye to monitor amplification using a real-time PCR cycler?

EvaGreen dye (Cat. No. 31000-T, Biotium Inc.) is recommended due to its high sensitivity and low interference with amplification chemistry. Depending on the real-time instrument used, a calibration dye may also be needed. Please refer to your real-time PCR instrument's user manual for additional information.

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