What is the recommended fluorescent dye to monitor amplification using a real-time PCR cycler?
What is the recommended fluorescent dye to monitor amplification using a real-time PCR cycler?
EvaGreen dye (Cat. No. 31000-T, Biotium Inc.) is recommended due to its high sensitivity and low interference with amplification chemistry. Depending on the real-time instrument used, a calibration dye may also be needed. Please refer to your real-time PCR instrument's user manual for additional information.