Is it necessary to perform quantification of ThruPLEX DNA-Seq libraries before and after the AMPure XP cleanup step?
Is it necessary to perform quantification of ThruPLEX DNA-Seq libraries before and after the AMPure XP cleanup step?
No, it is only necessary to quantify your individual or pooled libraries prior to sequencing. If you choose to quantify your libraries after the Library Amplification Reaction, it is recommended to do a final quantification of the purified libraries prior to sequencing because AMPure XP purification can result in loss of DNA.