How deep should I sequence Seeker libraries?
How deep should I sequence Seeker libraries?
- Although we recommend 2 x 108–6 x 108 paired-end reads for the 3 mm x 3 mm tile and 2 x 108–3 x 108 billion paired-end reads for the 10 mm x 10 mm tile, deeper sequencing may be required for more transcriptionally active tissue types. In the HTML
- Seeker Primary Analysis Pipeline, the metric "median reads/UMI" indicates the approximate sequencing saturation. We recommend a value of 8–10 to reach saturation. If it is lower than this range, we recommend sequencing more to recover additional UMIs.