How much PhiX should I spike in to my library before sequencing?
How much PhiX should I spike in to my library before sequencing?
- We recommend using the same recommendations as those for the NovaSeq 6000, as outlined in the user guide.
Phi-X spike-in recommendations:
- NextSeq 1000/2000: 5% PhiX spike-in
- NextSeq 500/550: 5% PhiX spike-in
- NovaSeq 6000/X Plus:
- 5% PhiX spike-in when pooling with non-Seeker libraries
- 10% PhiX spike-in when sequencing only Seeker libraries