What buffer should I collect cells in for compatibility with direct SMARTer Stranded Total RNA-Seq Kit v3 Pico cDNA synthesis?
What buffer should I collect cells in for compatibility with direct SMARTer Stranded Total RNA-Seq Kit v3 Pico cDNA synthesis?
SMARTer Stranded Total RNA-Seq Kit v3 Pico cDNA synthesis has been validated with cell collected or resuspended in Ca/Mg-free PBS. The final volume of collected cells should not exceed the sample input volume recommended in the SMARTer® Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian User Manual.